Methicillin Resistant Staphylococcus aureus (MRSA) by PCR
| Order Code: | MRSAPCR |
| Epic Lab Code: | LAB5810 |
| Order Form: | A-1a Clinical Microbiology Laboratory or Epic Req |
Microbiology
BT 6004
356-2591
Bacteriology/Virology Section
BT 6004
356-2591
Bacteriology/Virology Section
Specimen:
Surveillance Swab Specimen collected from Nares
Rejection Criteria:
Specimen collected using different swab (not Copan dual swab) or from
site other than nares (culture will be set up instead).
Specimen
Instructions:
Copan Dual Swab (#26200) is available from Hospital Stores.
With patient's head tilted back, insert both dry swabs (leave attached
to red cap) approximately 1-2 cm into one nostril. Rotate the swabs
against the inside of the nostril for 3 seconds. Apply slight pressure
with a finger on the outside of the nose to ensure good contact between
the swabs and inside of the nose. Using the same swabs, repeat for the
other nostril. Place the swabs into the transport tube (swabs should
stay attached to the red cap). Make sure the red cap is tightly on the
transport tube.
Transport: Room temperature up to 24 hours, otherwise refrigerate
(2-8 degrees C) up to 5 days.
Testing Schedule:
24 hrs/day, 7 days a week, including holidays.
Analytic Time:
2 hours (upon receipt in laboratory)
Comments:
Used to detect colonization with methicillin-resistant Staphylococcus
aureus (MRSA) only. A positive result does not necessarily indicate
viable organism.
The primers and probes in the Xpert MRSA assay detect a proprietary
sequence for the presence of a cassette inserted into the S. aureus
chromosome. Mutations or polymorphisms in primer or probe binding
regions may affect detection of new or unknown MRSA variants resulting
in a false negative result. Limit of detection observed among the
SCCmec cassette types ranged from 10-100 CFU/swab [types I, II, III, V
(10 CFU/swab), type IV (50 CFU/swab), subtype IVa (100 CFU/swab)].
Reliable results are dependent on proper specimen collection, handling,
and storage.
Methodology:
Real-time polymerase chain reaction
CPT Code:
87641
