KRAS Mutational Analysis
| Epic Lab Code: | LAB5763 |
| Order Form: | A-1a Miscellaneous Request or Epic Req |
Molecular Pathology
6004 BT GH
384-9568
6004 BT GH
384-9568
Specimen:
Formalin fixed, paraffin embedded tissue block and 10 unstained 5-10 μM slides at 20-25°C and an H&E slide of the tissue
Minimum:
Tumor cells more than 50% of the total tissue and greater than
10mm2 in surface area on the block.
Rejection Criteria:
Specimens fixed in B5 fixative or that have been decalcified will not
be accepted. Tumor specimens containing less than 50% tumor cells or
are less than 10mm2 in area may be unacceptable.
Testing Schedule:
Weekly
Analytic Time:
7-10 working days
Reference Range:
Negative for mutations (wild type).
Comments:
Order Code: KRAS
The tissue should be reviewed by a pathologist prior to testing to identify that it contains at least 50% tumor.
This assay detects mutations in codons 12, 13 and 61 (19 mutations in total).
The tissue should be reviewed by a pathologist prior to testing to identify that it contains at least 50% tumor.
This assay detects mutations in codons 12, 13 and 61 (19 mutations in total).
Methodology:
Polymerase Chain Reaction and Single Nucleotide Primer Extension
CPT Code:
88381, 83907, 83891, 83900, 83914 x7, 83912
Additional CPT code modifiers may be required for procedures to be performed to test oncologic or inherited disorders.
Additional CPT code modifiers may be required for procedures to be performed to test oncologic or inherited disorders.