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| KRAS Mutation Analysis | ||
| Order Code: KRAS
Epic Lab Code: LAB5763 Order Form: A-1a Miscellaneous Request or Epic Req |
Commercial "Mail-out" Laboratory 6240 RCP 356-3527 |
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Specimen: |
Formalin-fixed Tissue Block | ||
Collection Medium: |
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Minimum: |
Formalin-fixed, Paraffin-embedded Block with Corresponding H&E slide (preferred specimen type): Tissues should be well-fixed in formalin. If an alternative fixative is used, it should be noted on the requisition. Alternative Specimens: Unstained Slides or Fresh Snap Frozen Biopsy. Unstained Slides: *Send all slides within 5-7 days of cutting. *Minimum of 4 slides (plus 1 H&E) or 5 slides (w/o H&E). *Pre-cut slides from paraffin block in 7 micron sections. *Air dry. Do not oven dry. *Store specimen at room temperature (20-23.5 degrees C). | ||
Analytic Time: |
2 weeks | ||
Reference Range: |
See report. | ||
Interpretive Data: |
KRAS Mutation Analysis provides additional guidance in therapeutic treatment decisions for patients with colorectal cancer (CRC) and non-small cell lung cancer (NSCLC). Additional tumor types can include: Ovarian, Bile Duct, Pancreas, Esophageal, Appendix, Breast, Endometrial, Gastric, Thyroid. Mutations in the KRAS gene, a known downstream signaling molecule in the EGFR signaling pathway, have been described in approximately 30-50% of colorectal carcinomas and approximately 15-30% of lung adenocarcinomas. Recent studies have found mutations in the KRAS gene to be associated with a poor prognosis. Studies have also found KRAS mutations more frequent in patients who show limited clinical response to targeted anti-epidermal growth factor receptor (anti-EGFR) therapies. As a result, determining the KRAS mutational status of a tumor may guide therapeutic decision making for patients with CRC and NSCLC. The molecular diagnostic procedure incorporates allele-specific primer extensions for detecting mutations in codons 12 and 13 of the KRAS gene. | ||
Comments: |
Contact Commercial Mailouts at 356-8593 to obtain testing initiation, or fax a miscellaneous A-1a to our area at 353-6130 with the S# (surgical number). Mailouts will work in conjunction with Anatomic Pathology to have slides cut for testing sent to the reference laboratory. | ||
CPT Code: |
83890, 83892(x2), 83898, 83904(x2), 83907, 83909(x2), 83912 |
Updated: 09/15/2008
Note: The information contained in this handbook is for use by personnel of University of Iowa Health Care. No other use is implied or intended.