E. Coli Shiga toxin by EIA
Order Code: C EHEC
Order Form: A-1a Clinical Microbiology Laboratory
Microbiology
BT 6004
356-2591
Bacteriology/Virology Section
Specimen:
Stool
Collection Medium:
Sterile container
Minimum:
Submit 10-20 g stool in sterile container.
Delivery Instructions:
Transport time is less than or equal to 1 hr. Refrigerate immediately if transport is delayed.
Specimen Instructions:
Label specimen with two patient identifiers, date and time of collection.
Testing Schedule:
0700-2200, 7 days a week, including holidays.
Reference Range:
No Shiga-toxin-detected.
Comments:
This assay is a rapid in vitro microwell EIA for the detection of Shiga toxins I and II (verotoxins) in stool specimens. The test is intended for use as an aid in the diagnosis of enterohemorrhagic Escherichia coli (EHEC) infection. Shiga toxin testing of stool specimens provides better sensitivity than standard culture methods for the E. coli O157:H7 serotype and non-O157 serotypes. Testing of broth cultures, rather than the stool specimens themselves, is performed because the amount of free fecal Shiga toxin in stools is often low. All Shiga toxin positive broths (or presumptive E. coli O157:H7 isolates) will be forwarded to the University Hygienic Laboratory for confirmatory testing and genetic characterization. Shiga toxin testing is included in routine stool cultures as recommended by Centers for Disease Control (CDC).
Methodology:
Enzyme Immunoassay
CPT Code:
87427
 
See Additional Information:
Microbiology Specimen Collection and Transport