The University of Iowa (UIHC)
Department of Pathology
LABORATORY SERVICES HANDBOOK


Fluorescence In-Situ Hybridization (FISH-Paraffin Embedded Tumor)

  Cytogenetics Laboratory (Dept. of Pediatrics)
W-101 GH
356-3877 (Laboratory)
Specimen
Paraffin Embedded Tumor Tissue
Minimum:
4 unstained slides on charged slide, 1 H&E slide with tumor marked.  
All slides from the same block cut sequentially.
Delivery Instructions:
Submit specimen to laboratory as soon as possible after collection.
Testing Schedule:
Specimens are accepted Monday - Friday, 0800-1700.  Provide details of
clinical information.  If the specimen is collected over the weekend,
please page the technologist on call by dialing 1-888-533-0186.  When 
it stops ringing, enter your phone number, the '#' sign, and hang up.
Analytic Time:
Final results within 1 week.
Reference Range:
Male:  X and Y probe signals          Female:  two X probe signals
For autosomes, the reference range varies for each probe (set).  
Suitable for interphase and metaphase analysis.  Call lab for 
information.
Comments:
This test can be used for screening of sex chromosomes, for monosomies 
in cases such as glioma (eg.1p, 5, 7,19q, etc.), for trisomies (eg., 
+4, +8, +11, +12, +13, +14, +18, +19, +21, +22, etc.) or for detection 
of the gene fusion resulting from Translocations (such as IgH/MYC, 
BCR/ABL, Igh/CCND1, IgH/BCL2) in solid tumors.

If the desired FISH probe is not listed, call the laboratory.


Cytogenetics Laboratory Web Site
CPT Code:
88239, 88271, 88275, 88365
 
See Additional Information:
Cytogenetics Testing

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Updated: 02/18/2008

Note: The information contained in this handbook is for use by personnel of University of Iowa Health Care. No other use is implied or intended.