Bordetella Pertussis Ab, IgG & IgM by ELISA with Reflex to Immunoblot
Order Code: BORDPAN
Epic Lab Code: LAB4803
Order Form: A-1a Miscellaneous Request or Epic Req
Commercial Mail-out Laboratory
01250 PFP
356-3527
Specimen:
Serum
Collection Medium:
Red top tube
Minimum:
Preferred minimum: 1 mL serum Absolute minimum: 0.3 mL serum
Rejection Criteria:
Severely lipemic, contaminated, heat-inactivated, or hemolyzed specimens.
Specimen Instructions:
Acute and convalescent specimens must be labeled as such; parallel testing is preferred and convalescent specimens must be received within 30 days from receipt of the acute specimens. Please mark specimens plainly as "acute" or "convalescent."
Turn Around Time:
8 working days upon receipt at reference laboratory
Reference Range:
Bordetella pertussis Antibody, IgG by ELISA 0.9 U/mL or less: Negative - No significant level of detectable Bordetella pertussis IgG antibody. 1.0-2.4 U/mL: Equivocal - Repeat testing in 10-14 days may be helpful. 2.5 U/mL or greater: Positive - IgG antibody to Bordetella pertussis detected, which may indicate a current or past exposure/immunization to B. pertussis. Bordetella pertussis Antibody, IgG by Immunoblot Negative Bordetella pertussis Antibody, IgM by ELISA 0.9 U/mL or less: Negative - No significant level of detectable Bordetella pertussis IgM antibody. 1.0-1.1 U/mL: Equivocal - Repeat testing in 10-14 days may be helpful. 1.2 U/mL or greater: Positive - IgM antibody to Bordetella pertussis detected, which may indicate a current or recent exposure/ immunization to B. pertussis. Bordetella pertussis Antibody, IgM by Immunoblot Negative
Interpretive Data:
Recommend that treatment decisions be based on the result of the B. pertussis IgM immunoblot test instead of the ELISA test. B. pertussis IgM test by ELISA may produce false-positive results.
Comments:
Note: If Bordetella pertussis Antibody, IgG by ELISA is 2.5 U/mL or greater, then Bordetella pertussis IgG Immunoblot testing will be added; if Bordetella pertussis Antibody, IgM by ELISA is 1.2 U/mL or greater, then Bordetella pertussis IgM Immunoblot testing will be added. Additional charges apply.
Methodology:
Enzyme-Linked Immunosorbent Assay/Qualitative Immunoblot
CPT Code:
86615 x2 Bordetella (IgG & IgM); if reflexed add 86615 for each Bordetella Immunoblot