The University of Iowa (UIHC)
Department of Pathology
LABORATORY SERVICES HANDBOOK


MLH1/MSH2 Mutation Screen
Order Code: MLH1/MSH2
Order Form: A-1a Miscellaneous Request or IPR Req
  Commercial "Mail-out" Laboratory
6240 RCP
356-3527
Specimen
Whole Blood
Collection Medium:
Lavender top tube (EDTA)
Alternate
Collection Media:
Yellow top tube (ACD solution A)
Minimum:
Draw blood in a lavender-top (EDTA) tube(s) or a yellow-top (ACD)
tube(s), and send 3.0 mL of EDTA or ACD whole blood in original 
VACUTAINER(S).
Delivery Instructions:
Specimen must arrive commerical laboratory within 96 hours of draw.
Analytic Time:
within 10 days
Reference Range:
An interpretive report will be provided.
Comments:
Extracted DNA is also an appropriate sample type.
Methodology:
DNA sequencing and dosage analysis are utilized to test for the 
presence of a mutation in all 19 exons of the hMLH1 gene and all 16 
exons of the hMSH2 gene Polymerase chain reaction (PCR).
CPT Code:
DNA Sequence Analysis:  83891, 83892(x4), 83900, 83901 (x2),
                        83909 (x70), 83912

Gene Dosage Analysis:   83896, 83901, 83909

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Updated: 06/20/2007

Note: The information contained in this handbook is for use by personnel of University of Iowa Health Care. No other use is implied or intended.