Neutrophil Oxidative Burst Assay (DHR), Whole Blood + Control Whole Blood

Neutrophil Oxidative Burst Assay (DHR)

Order Code:	DHR
Epic Lab Code:	LAB3710

Commercial Mail-out Laboratory
6240 RCP
356-3527

Specimen:

Whole Blood + Control Whole Blood

Collection Medium:

	plus control
Green top tube 4 mL (Na Heparin)	plus control	Green top tube 4 mL (Na Heparin)

Minimum:

Adult/Pediatric Preferred Minimum: One 4 mL green (sodium heparin) and one normal control in a 4 mL green (sodium heparin) from a healthy unrelated individual.

Rejection Criteria:

Refrigerated or frozen samples or samples in transport longer than 48 hours.

Delivery Instructions:

CRITICAL AMBIENT. Do not refrigerate or freeze.

Specimen Instructions:

Patient and control samples must be collected within 48 hours of test performance.

Analytic Time:

3 working days upon receipt at reference laboratory

Reference Range:

By report

Interpretive Data:

White blood cells are incubated with dihydrorhodamine 123 (DHR) and catalase, then stimulated with Phorbol 12-Myristate 13-Acetate (PMA). Dihydrorhodamine oxidation to rhodamine by the respiratory burst of the cell is measured by flow cytometry. Results are reported as the ratio of the mean channel fluorescence of stimulated cells versus unstimulated cells, which yields a stimulation index (SI).

Comments:

If sample shows abnormal results when stimulated, and no control was sent, test should be resubmitted with control sample to validate the conditions of collection, processing and transport.

Methodology:

Flow Cytometry

CPT Code:

86352