HLA Genotyping A, B or C Class I - Intermediate Resolution (VAMC)
Iowa Regional Histocompatibility and Immunogenetics
Veterans Affairs Hospital 10E-19
319-338-0581, EXT. 5640
(dial 158 from UIHC)
Specimen:
Whole Blood
Minimum:
THREE - FOUR 10 mL yellow top (ACD) tubes or purified genomic DNA. For patients with low white counts-additional tubes are needed. Buccal swabs may be used if normal sample requirements can not be met.
Delivery Instructions:
Deliver at room temperature.
Testing Schedule:
Test performed twice weekly.
Analytic Time:
Resulted in Epic 4-5 working days.
Comments:
Order each allele separately.

All HLA Testing is ordered through the University of Iowa Epic System.
Methodology:
Polymerase Chain Reaction(PCR) - Sequence Specific Oligonucleotide (SSO) or Sequence Specific Primers (SSP)
CPT Code:
83891(x1) each 83900(x1) each 83912(x1) each 83896(x74) A 83896(x100) B 83896(x58) C For HLA-A use modifier (4A) HLA-B use modifier (4B) HLA-C use modifier (4C)
 
See Additional Information:
Iowa Regional Histocompatibility and Immunogenetics Laboratory Required Content on Requisitions