The University of Iowa (UIHC)
Department of Pathology
LABORATORY SERVICES HANDBOOK

Quantitative STR CD3 Subset Analysis (VAMC)

Order Form: Tissue Typing Laboratory Test Requisition 		  Iowa Regional Histocompatibility and Immunogenetics
Veterans Affairs Hospital 10E-19
(319-338-0581), EXT. 5640
dial 158 from UIHC 
Specimen
 
Whole Blood
Minimum:
 
THREE - FOUR 10 mL yellow top (ACD) tubes.
Delivery Instructions:
 
Deliver at room temperature.
Analytic Time:
 
7 days
Comments:
 
Requires pre-transplant donor sample AND pre-transplant recipient 
sample and prior Qualitative STR testing.

Engraftment test provides percentage of donor DNA in purified CD3 
subset from post-transplant recipient blood sample by quantitative 
analysis of 1 STR locus.
Methodology:
 
Polymerase Chain Reaction (PCR), magnetic cell separation, flow 
cytometry and Sequence Based Typing (SBT).
CPT Code:
 
83891(x3), 83909(x9), 83900(x1), 83901(x52), 83912(x2), 86849(x1),
88184(x1), 88185(x2), 88187(x1)
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Updated: 02/28/2008

Note: The information contained in this handbook is for use by personnel of University of Iowa Health Care. No other use is implied or intended.