Quantitative STR CD3 Subset Analysis (VAMC)
Iowa Regional Histocompatibility and Immunogenetics
Veterans Affairs Hospital 10E-19
319-338-0581, EXT. 5640
(dial 158 from UIHC)
Specimen:
Whole Blood
Minimum:
THREE - FOUR 10 mL yellow top (ACD) tubes.
Delivery Instructions:
Deliver at room temperature. Sample must arrive by 11:00 a.m.
Testing Schedule:
Test performed daily.
Analytic Time:
Results in Epic by 5-7 working days.
Comments:
Requires pre-transplant donor sample AND pre-transplant recipient sample and prior Qualitative STR testing. Engraftment test provides percentage of donor DNA in purified CD3 subset from post-transplant recipient blood sample by quantitative analysis of 1 STR locus. All HLA Testing is ordered through the University of Iowa Epic System.
Methodology:
Polymerase Chain Reaction (PCR), magnetic cell separation, flow cytometry and Sequence Based Typing (SBT).
CPT Code:
83891(x1), 83909(x5), 83900(x1), 83901(x43), 83912(x2), 86849(x1), 88184(x1), 88185(x2), 88187(x1) Use modifier (4I or 4L)
 
See Additional Information:
Iowa Regional Histocompatibility and Immunogenetics Laboratory Required Content on Requisitions