HLA Genotyping DRB1, DRB3, 4, 5 or DQ Alpha Beta Intermediate Resolution (VAMC)
Iowa Regional Histocompatibility and Immunogenetics
Veterans Affairs Hospital 10E-19
319-338-0581, EXT. 5640
(dial 158 from UIHC)
Specimen:
Whole Blood
Minimum:
THREE - FOUR 10 mL yellow top (ACD) tubes. For patients with low white counts-additional tubes are needed. Buccal swabs may be used if normal sample requirements can not be met.
Delivery Instructions:
Deliver at room temperature.
Testing Schedule:
Test performed twice weekly.
Analytic Time:
Resulted in Epic by 5 working days.
Comments:
Order each allele separately.

All HLA Testing is ordered through the University of Iowa Epic System.
Methodology:
Polymerase Chain Reaction (PCR) - Sequence Specific Oligonucleotide (SSO)
CPT Code:
86891(x1) each 83900(x1) each 83912(x1) each 83896(x70) DRB1 Use modifier (4P) 83896(x65) DRB3, 4, 5 Use modifier B3 use 4Q, B4 use 4R, B5 use 4S 83896(x100) DQB1/Alpha Use modifier DQB use 4F, DQA use 4T
 
See Additional Information:
Iowa Regional Histocompatibility and Immunogenetics Laboratory Required Content on Requisitions