The University of Iowa (UIHC)
Department of Pathology
LABORATORY SERVICES HANDBOOK

Hepatitis B Virus (HBV) Lamivudine Resistance, RFLP
Order Code: HEPBLAM
Order Form: A-1a Miscellaneous Request or IPR Req 		  Commercial "Mail-out" Laboratory
6240 RCP
356-3527 
Specimen
 
Plasma
Collection Medium:
Lavender top tube (EDTA) 


Alternate
Collection Media:


Pink top tube (EDTA sprayed), Yellow top tube (ACD solution A)


Minimum:


4 mL into two 2 mL aliquots


Rejection Criteria:


Serum


Analytic Time:


within 10 days


Reference Range:


By report


Test
Limitations:


Mutations at codons 204 and 180 of the HBV DNA polymerase gene are 
associated with a diminished response to the antiviral drug lamivudine. 
For successful analysis, specimens should have a HBV viral load of at 
least 500 copies/mL.  Patient must have viral load within 30 days for 
testing to be performed.


Methodology:


Line Probe Assay (LIPA)


CPT Code:


83890, 83894, 83896, 83898 (x2), 83912




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Updated: 03/28/2008

Note: The information contained in this handbook is  
            for use by personnel of University of Iowa Health Care. 
            No other use is implied or intended.