Hepatitis B Virus (HBV) Lamivudine Resistance, RFLP, Plasma

Hepatitis B Virus (HBV) Lamivudine Resistance, RFLP

Order Code:	HEPBLAM
Epic Lab Code:	LAB3305
Order Form:	A-1a Miscellaneous Request or Epic Req

Commercial Mail-out Laboratory
6240 RCP
356-3527

Specimen:

Plasma

Collection Medium:

	and
Lavender top tube 3 mL (EDTA)	and	Lavender top tube 3 mL (EDTA)

Alternate Collection Media:

Pink top tube, Yellow top tube (ACD solution A)

Minimum:

4 mL into two 2 mL aliquots

Rejection Criteria:

Serum

Analytic Time:

within 10 days upon receipt at reference laboratory

Reference Range:

By report

Test Limitations:

Mutations at codons 204 and 180 of the HBV DNA polymerase gene are associated with a diminished response to the antiviral drug lamivudine. For successful analysis, specimens should have a HBV viral load of at least 500 copies/mL. Patient must have viral load within 30 days for testing to be performed.

Methodology:

Line Probe Assay (LIPA)

CPT Code:

83890, 83894, 83896, 83898 (x2)