JAK2 V617F Mutation Detection Assay, Whole Blood

JAK2 V617F Mutation Detection Assay

Order Code:	JAK2
Epic Lab Code:	LAB2472
Order Form:	A-1a Molecular Pathology/Diagnostics or Epic Req

Molecular Pathology
6004 BT GH
384-9568

Specimen:

Whole Blood

Collection Medium:

Lavender top tube 3 mL (EDTA)

Minimum:

Adults 3 mL whole blood. Testing on smaller volumes than those requested will be attempted. However, in some cases, small blood volumes may compromise the ability to perform testing. Testing requires a dedicated collection tube.

Delivery Instructions:

Room temperature for up to 24 hours, then refrigerate the whole blood if it is necessary to be held overnight, or weekends, or holidays.

Testing Schedule:

Weekly

Analytic Time:

7 working days

Reference Range:

Direct detection of G-T tranversion at position 1849 of the Janus kinase 2 locus, giving rise to a Valine-to-Phenylalanine substitution at position 617 (JAK2 V617F mutation).

Comments:

This test is useful for diagnosis of myeloproliferative isorders: Polycythemia Vera, Essential Thrombocythemia and Chronic Idiopathic Myelofibrosis.

Methodology:

Allele specific Polymerase Chain Reaction followed by fluorescence capillary electrophoresis

CPT Code:

83909, 83900, 83890, 83912