Specimen

Whole Blood

Collection Medium:

Minimum:

Adults 3 ml whole blood.  Testing on smaller volumes than those 
requested will be attempted. However, in some cases, small blood 
volumes may compromise the ability to perform testing.  Testing 
requires a dedicated collection tube.

Delivery Instructions:

Room temperature for up to 24 hours, then refrigerate 
the whole blood if it is necessary to be held overnight, 
or weekends, or holidays.

Testing Schedule:

Weekly

Analytic Time:

7 working days

Reference Range:

Direct detection of G-T tranversion at position 1849 of the Janus 
kinase 2 locus, giving rise to a Valine-to-Phenylalanine substitution 
at position 617 (JAK2 V617F mutation).

Comments:

This test is useful for diagnosis of myeloproliferative isorders: 
Polycythemia Vera, Essential Thrombocythemia and Chronic Idiopathic 
Myelofibrosis.

Methodology:

Allele specific Polymerase Chain Reaction followed by fluorescence 
capillary electrophoresis

CPT Code:

83909, 83900, 83890, 83912