Microsatellite Instability
| Epic Lab Code: | LAB1980 |
| Order Form: | A-1a Molecular Pathology/Diagnostics or Epic Req |
Molecular Pathology
6004 BT GH
384-9568
6004 BT GH
384-9568
Specimen:
Formalin fixed, paraffin embedded tissue block and 10 unstained 5-10 μM slides at 20-25°C and an H&E slide of the tissue
Minimum:
Tumor cells more than 50% of the total tissue and greater than
10mm2 in surface area on the block.
Must also submit a healthy tissue specimen or peripheral blood from the same subject for comparison of microsatellite markers.
Must also submit a healthy tissue specimen or peripheral blood from the same subject for comparison of microsatellite markers.
Rejection Criteria:
Specimens fixed in B5 fixative or that have been decalcified will not
be accepted. Tumor specimens containing less than 50% tumor cells or
are less than 10mm2 in area may be unacceptable.
Testing Schedule:
Weekly
Analytic Time:
7 working days
7-10 working days
Reference Range:
Microsatellite Stable
Comments:
Order Code: MSI
Tumor tissue and normal tissue should be obtained as part of the surgical procedure for testing. The tissue should be formalin-fixed, paraffin embedded and reviewed by a surgical pathologist prior to MSI testing. A block containing normal tissue (non-tumor) should be submitted for testing in addition to a block containing at least 50% tumor. Acceptable tissue for the non-tumor block is tissue with a high nuclear content, preferably benign lymph node tissue if available. Blood submitted in EDTA or sodium citrate is acceptable for the normal block if tissue is not available. An H&E stained glass slide from both blocks should also be submitted.
Tumor tissue and normal tissue should be obtained as part of the surgical procedure for testing. The tissue should be formalin-fixed, paraffin embedded and reviewed by a surgical pathologist prior to MSI testing. A block containing normal tissue (non-tumor) should be submitted for testing in addition to a block containing at least 50% tumor. Acceptable tissue for the non-tumor block is tissue with a high nuclear content, preferably benign lymph node tissue if available. Blood submitted in EDTA or sodium citrate is acceptable for the normal block if tissue is not available. An H&E stained glass slide from both blocks should also be submitted.
Methodology:
Multiplex PCR followed by Fluorescence Capillary Electrophoresis
CPT Code:
83907(X2), 83909(X2), 83900, 83901, 83912