The University of Iowa (UIHC)
Department of Pathology
LABORATORY SERVICES HANDBOOK


Organ-Tissue Donor Panel (Marrow and Stem Cell Donors only)
Order Code: ORGTMN
Order Form: A-1a Miscellaneous Request or IPR Req
  Commercial "Mail-out" Laboratory
6240 RCP
356-3527
Specimen
Whole Blood and Serum
Minimum:
2 full 6 mL pink K2 EDTA top tube
1 full 6 mL red top tube
Analytic Time:
1 week
Reference Range:
CMV Antibody
  Negative (Positive result suggest current or past infection with
  Cytomegalovirus (CMV).  The presence of Anti-CMV does not assure
  immunity to the disease.)
Hepatitis B Core Antigen
  Negative
Hepatitis B Surface Antigen
  Non-reactive
Hepatitis C Antibody
  Non-reactive
HIV 1/2 Antibody plus O
  Non-reactive
HIV-1/HCV NAT
  Non-reactive
HTLV I/II Antibody Screen
  Non-reactive
Rapid Plasmin Reagin (RPR)
  Non-reactive
West Nile Virus (WNV NAT)
  Negative
Methodology:
CMV antibody, Hepatitis B Core Antigen, Hepatitis B Surface Antigen, 
Hepatitis C Antibody, HIV 1/2 Antibody plus O, CMV-Immucor and HTLV 
I/II Antibody Screen use Enzyme Immunoassay (EIA) methodology for 
testing.

HIV-1/HCV NAT and West Nile Virus (NAT) use Nucleic Acid Testing (NAT) 
Roche Molecular method.

Rapid Plasmin Reagin uses RPR Latex agglutination.

Confirmatory Testing:
Hepatitis B Surface Antigen Confirmation:  Ortho Enzyme Immunoassay 
System 3.0
Hepatitis C Antibody Confirmation:  Recombinant Immunoblot Assay (HCV 
RIBA:  Chiron-SIA-v.3.0)
HIV 1/2 Antibody Immunoblot will be confirmed on any reactive specimen.  
Confirmatory scheme includes the following testing:  Anti HIV-1 Western 
Blot (Bio-Rad Western blot), HIV-2 EIA (if Western blot negative) 
(Bio-Rad), and HIV-2 Immunoblot (if HIV-2 EIA positive) (Viromed).

Rapid Plasmin Reagin (RPR) confirmatory testing:  Fluorescent 
Treponemal Antibody - ABS (confimatory testing sent to Viromed)
CPT Code:
CMV Antibody CPT = 86644

Hepatitis B Core Antigen  CPT = 86704

Hepatitis B Surface Antigen  CPT = 87340
  (Hepatitis B Surface Antigen Confirmatory testing done
   automatically if positive.  CPT code =  87340)

Hepatitis C Antibody CPT = 86803
  (Hepatitis C Antibody Confirmatory testing done automatically
   if positive.  CPT code = 86804)

HIV 1/2 Antibody plus O CPT = 86703
  (HIV 1/2 Antibody Immunoblot will be confirmed on any reactive
   specimen; CPT code = 86689.  Confirmatory scheme includes the
   following testing:  Anti HIV-1 Western Blot (Bio-Rad),
   HIV-2 EIA (if Western blot negative--Bio-Rad), and
   HIV-2 Immunoblot (if HIV-2 EIA positive--Viromed).

HIV-1/HCV NAT testing: HIV-1 CPT code = 87535; HCV CPT code = 87521

HTLV I/II Antibody:  HTLV I CPT code = 86687; HTLV II CPT code =
  86688.  HTLV I/II virus type I/II antibody confirmation will be
  performed to confirm presence of antibodies to HTLV-I HTLV-II in
  samples repeatedly reactive by EIA screening test.  Second
  manufacturer's EIA method CPT codes = 86687 and 86688.

Rapid Plasmin Reagin (RPR) CPT code = 86592  If positive, confirmatory
  test is performed FTA-ABS = 86781 at Viromed.

West Nile Virus (WNV NAT) CPT code = 87798

Alphabetic main page

Updated: 06/06/2008

Note: The information contained in this handbook is for use by personnel of University of Iowa Health Care. No other use is implied or intended.