Fluorescence In-Situ Hybridization (FISH-Aneuploidy Screening)

Label Mnemonic:	ANEUP
Downtime form:	C-12 Cytogenetics Request

Shivanand R. Patil Cytogenetics & Molecular Laboratory
Department of Pediatrics
W-101 GH
356-3877 (Laboratory)

Specimen(s):

Paraffin Embedded Tumor Tissue or Peripheral Blood (Newborn or Cord, and Others)

Minimum:

4 sections cut at 2 microns and an accompanying H&E side from the tissue block with the most villi.

2 cc of infants venous blood collected in a green-top Vacutainer^® with sodium heparin. Invert tube to mix well. Label the tube with patient name and medical record number. DO NOT FREEZE OR CENTRIFUGE.

Delivery Instructions:

Submit specimen to laboratory as soon as possible after collection. Provide details of clinical information and family history (if applicable). After hours specimens should be taken to Specimen Control. For questions after hours, call (319) 356-1616 and ask the operator to page the cytogenetics on-call staff at pager #5525.

Testing Schedule:

Specimens accepted in the lab Monday-Friday, 0800-1700.

Turn Around Time:

Preliminary results are given for STAT blood cases within 48 hours. Suitable for interphase and metaphase analysis. Allow 2-5 days for final results. Preliminary results are not provided for paraffin embedded tumor tissue samples.

Reference Range:

Male:  X and Y probe signals          Female:  two X probe signals
The reference range for autosomes varies for each probe, call lab for 
information.

Comments:

Epic code: CYT54

Shivanand R. Patil Cytogenetics & Molecular Laboratory Website

Methodology:

Fluorescence In-Situ Hybridization (FISH)

CPT Code:

88271 x5, 88275 x5, 88291

See Additional Information:
Cytogenetics and Molecular Testing