Fluorescence In-Situ Hybridization (FISH-Bone Marrow)

Downtime form:

C-12 Cytogenetics Request

Shivanand R. Patil Cytogenetics & Molecular Laboratory
Department of Pediatrics
W-101 GH
356-3877 (Laboratory)

Specimen(s):

Bone Marrow, Hematologic Malignancies, Lymph Node, Fine-Needle Aspirate, Pleural Effusion, CSF

Specimen Instructions:

Provide details of clinical information.

Minimum:

Marrow Aspirate: 1-2 cc of bone marrow collected in a bone marrow media tube available from our lab or in a green-top Vacutainer^® with sodium heparin. Invert tube to mix well. Label the tube with patient name and medical record number. DO NOT FREEZE OR CENTRIFUGE.

Lymph Node: piece of lymph node biopsy

Tumor Fluid: collect between 1-5cc fluid with good cellularity

Delivery Instructions:

Submit specimen to laboratory as soon as possible after collection. After hours specimens should be taken to Specimen Control. For questions after hours, call (319) 356-1616 and ask the operator to page the cytogenetics on-call staff at pager #5525. Provide details of clinical information.

Testing Schedule:

Specimens accepted in the lab Monday-Friday, 0800-1700.

Turn Around Time:

Allow 3-5 days for final results. Contact laboratory if STAT TAT required.

Reference Range:

Varies for each probe (set). Unique sequence probes labeled with fluorochrome. Contact lab for details.

Comments:

Epic code: CYT54

This test can be used as an adjunct to chromosomal analysis for detection of clonal abnormalities including residual disease. Suitable for interphase and metaphase analysis.

Shivanand R. Patil Cytogenetics & Molecular Laboratory Testing Menu

Methodology:

Fluorescence In-Situ Hybridization (FISH)

CPT Code:

88271 x # of probes
88275 x # of probes, 88291

See Additional Information:
Cytogenetics and Molecular Testing