Fluorescence In-Situ Hybridization (FISH-Hematological Blood)
Epic Lab Code: CYT54
Order Form: C-12 Cytogenetics Request or Epic Req
Shivanand R. Patil Cytogenetics & Molecular Laboratory (Dept. of Pediatrics)
W-101 GH
356-3877 (Laboratory)
Specimen:
Peripheral Blood
Minimum:
5-10 cc adult, 2 cc infants of venous blood collected in a green-top vacutainer with sodium heparin. Invert tube to mix well. Label the tube with patient name and medical record number. DO NOT FREEZE OR CENTRIFUGE.
Delivery Instructions:
Submit specimen to laboratory as soon as possible after collection.
Specimen Instructions:
Provide details of clinical information. If a specimen is collected over the weekend, please page the technologist on call by dialing 1-888- 533-0186. When it stops ringing, enter your phone number, the '#' sign, and hang up.
Testing Schedule:
Specimens accepted in the lab Monday-Friday, 0800-1700. After hours specimens should be taken to specimen control and a message left on the lab voice mail. In the case of an emergency, follow the instructions on the lab voice mail.
Analytic Time:
Allow 3-5 days for final results.
Reference Range:
Varies for each probe (set). Unique sequence probes labeled with fluorochrome. Contact lab for details.
Comments:
This test can be used as an adjunct to chromosomal analysis for detection of residual disease. Suitable for interphase and metaphase analysis. Probes currently available include: ALK [2p23], AML1/ETO [t(8;21)], BCR/ABL [t(9;22)], CBFB [inv (16)], Cep7/D7S522[-7 and del(7q31)], Cep8 [trisomy 8], Cep12 [trisomy 12], CepX/Y[transplants], CLL probe panel, D13S319[del(13q)], D13S25[del(13q)], EGR1[del(5q31)], IgH/BCL-2[t(14;18)], IgH/CCND1[t(11;14)], IgH/MYC[t(8;14)], MLL [del(11)(q23)], N-MYC [2p24.1 or for amplification 2p24.1], p53 [del(17)(p13.1)], PML/RARA [t(15;17)], Rb1 [del(13)(q14)], TEL/AML1 [t(12;21)]. If the desired FISH probe is not listed, call the laboratory. *CEP = centromere enumeration probe Shivanand R. Patil Cytogenetics & Molecular Laboratory Website
Methodology:
Fluorescence In-Situ Hybridization (FISH)
CPT Code:
88237, 88271, 88275, 88365
 
See Additional Information:
Cytogenetics and Molecular Testing