ANTINEURONAL NUCLEAR ANTIBODY-Type 1 (ANNA-1) Negative at <1:2 ANTINEURONAL NUCLEAR ANTIBODY-Type 2 (ANNA-2) Negative at <1:2 ANTINEURONAL NUCLEAR ANTIBODY-Type 3 (ANNA-3) Negative at <1:2 PURKINJE CELL CYTOPLASMIC ANTIBODY, Type 1 (PCA-1) Negative at <1:2 PURKINJE CELL CYTOPLASMIC ANTIBODY, Type 2 (PCA-2) Negative at <1:2 PURKINJE CELL CYTOPLASMIC ANTIBODY, Type Tr (PCA-Tr) Negative at <1:2 AMPHIPHYSIN ANTIBODY Negative at <1:2 CRMP-5-IgG Negative at <1:2 Note: Titers lower than 1:2 are detectable by recombinant CRMP-5 Western blot analysis. CRMP-5 Western blot analysis will be done on request on stored spinal fluid (held 4 weeks). This supplemental testing is recommended in cases of chorea, vision loss, cranial neuropathy and myelopathy. Call the Clinical Pathology Core Laboratory at 356-3527 to request CRMP-5 Western blot. Neuron-restricted patterns of IgG staining that do not fulfill criteria for the listed autoantibodies may be reported as "unclassified antineuronal IgG." If detected, newly identified autoantibody specificities may be reported. Complex patterns that include non-neuronal elements may be reported as "uninterpretable."

Testing Algorithm If IFA is indeterminate, paraneoplastic autoantibody Western blot is performed at an additional charge. If IFA pattern is suggestive of neuromyelitis optica (NMO), then NMO-IgG is performed at an additional charge. If client requests or if IFA patterns suggest CRMP-5-IgG, CRMP-5-IgG Western blot is performed at an additional charge. If IFA patterns suggest GAD65 antibody, GAD65 antibody radioimmunoprecipitation assay is performed at an additional charge. If IFA patterns suggest amphipysin antibody, amphiphysin Western blot is performed at an additional charge.

Indirect Immunofluorescence Assay (IFA) Radioimmunoprecipitation Assay (RIA) Western Blot

86256 AGNAC, 86256 Amphiphysin, 86256 ANNA-1, 86256 ANNA-2, 86256 ANNA-3, 86256 CRMP-5-IgG, 86256 PCA-1, 86256 PCA-2, 86256 PCA-Tr, 84182 Paraneoplastic autoantibody Western blot confirmation (if appropriate), 84182 CRMP5 Western blot confirmation (if appropriate), 86341 GAD65 confirmation (if appropriate) 84182 Amphiphysin Western Blot confirmation (if appropriate)