The University of Iowa (UIHC)
Department of Pathology
LABORATORY SERVICES HANDBOOK


Anti-Nuclear Antibody (ANA) Screen with Reflex Titer
Order Code: ANAS
Epic Lab Code: LAB1256
Order Form: A-1a Immunopathology or Epic Req
  Immunopathology
5238 RCP
356-2688
Specimen:
Serum
Collection Medium:
Red top tube
Minimum:
Adult and pediatric - 2 ml; red top tube
Testing Schedule:
Daily - Batch analysis performed daily excluding 
weekends and university holidays.
Analytic Time:
3 days
Reference Range:
<1:40 for screen and titer
Comments:
If the screen is positive at 1:40, ANA titer is automatic add-on.  Sera 
positive at 1:80 Dilution will be held for three weeks.
Sera positive at 1:320 or greater will be stored in serum bank for 1 
year if additional ENA testing is necessary.  ANA screen will detect 
centromere and most other specificities, but may not detect 
Anti-SS-A-(Ro)Antibody.
Methodology:
Indirect Immunofluorescence on Hep 2 cells
CPT Code:
86038 ANA screen
86039 ANA titer

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Updated: 03/24/2009

Note: The information contained in this handbook is for use by personnel of University of Iowa Health Care. No other use is implied or intended.