The University of Iowa (UIHC)
Department of Pathology
LABORATORY SERVICES HANDBOOK

Anti-Nuclear Antibody (ANA) Screen with Reflex Titer
Order Code: ANAS
Epic Lab Code: LAB1256
Order Form: A-1a Immunopathology or Epic Req 		  Immunopathology
5238 RCP
356-2688 
Specimen:
 
Serum
Collection Medium:
Red top tube 


Minimum:


Adult and pediatric - 2 ml; red top tube


Testing Schedule:


Daily - Batch analysis performed daily excluding 
weekends and university holidays.


Analytic Time:


3 days


Reference Range:


<1:40 for screen and titer


Comments:


If the screen is positive at 1:40, ANA titer is automatic add-on.  Sera 
positive at 1:80 Dilution will be held for three weeks.
Sera positive at 1:320 or greater will be stored in serum bank for 1 
year if additional ENA testing is necessary.  ANA screen will detect 
centromere and most other specificities, but may not detect 
Anti-SS-A-(Ro)Antibody.


Methodology:


Indirect Immunofluorescence on Hep 2 cells


CPT Code:


86038 ANA screen
86039 ANA titer




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Updated: 03/24/2009

Note: The information contained in this handbook is  
            for use by personnel of University of Iowa Health Care. 
            No other use is implied or intended.