The University of Iowa (UIHC)
Department of Pathology
LABORATORY SERVICES HANDBOOK


Phosphatase, Alkaline Isoenzymes
Order Code: ALPI
Order Form: A-1a Miscellaneous Request or IPR Req
  Commercial "Mail-out" Laboratory
6240 RCP
356-3527
Specimen
Serum
Collection Medium:
Red top tube
Alternate
Collection Media:
Green top tube (Na Heparin), Light Green top tube (Lithium Heparin)
Minimum:
Adult preferred minimum: 2 mL serum from red top tube
Adult absolute minimum: 1 mL serum from red top tube
Pediatric minimum: 0.5 mL serum from red top tube
Rejection Criteria:
Hemolyzed specimens
Analytic Time:
2 working days (upon receipt)
Reference Range:
Components:              Male                        Female
Alkaline Phos:   0-30 days: 60-320 U/L       0-30 days: 60-320 U/L
serum or plasma  1-11 months: 70-350 U/L     1-11 months: 70-350 U/L
                 1-3 years: 125-320 U/L      1-3 years: 125-320 U/L
                 4-6 years: 150-370 U/L      4-6 years: 150-370 U/L
                 7-9 years: 150-440 U/L      7-9 years: 150-440 U/L
                 10-11 years: 150-470 U/L    10-11 years: 150-530 U/L
                 12-13 years: 160-500 U/L    12-13 years: 110-525 U/L
                 14-15 years: 130-530 U/L    14-15 years: 55-305 U/L
                 16-19 years: 60-270 U/L     16-19 years: 40-120 U/L
                 20+ years: 40-120 U/L       20+ years: 40-120 U/L

Bone:            1-6 years: 0-208 U/L        1-6 years: 0-189 U/L
                 7-9 years: 0-264 U/L        7-9 years: 0-246 U/L
                 10-15 years: 0-340 U/L      10-13 years: 0-340 U/L
                 16-19 years: 0-165 U/L      14-15 years: 0-91 U/L
                 20+ years: 0-55 U/L         16+ years: 0-55 U/L

Liver:           1-6 years: 0-145 U/L        1-9 years: 0-148 U/L
                 7-11 years: 0-182 U/L       10-15 years: 0-162 U/L
                 12-15 years: 0-226 U/L      16+ years: 0-94 U/L
                 16-19 years: 0-114 U/L
                 20+ years: 0-94 U/L
Comments:
Fasting sample is recommended.

Note:  Heat-stable isoenzymes, such as the one produced during 
pregnancy, will interfere with the interpretation of this assay.
Test
Limitations:
Bone Specific Alkaline Phosphatase and 5'nucleotidase may be useful in 
identifying disorders of bone and liver, respectively.
Methodology:
Heat Inactivation/Enzymatic
CPT Code:
84075, 84080

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Updated: 05/23/2007

Note: The information contained in this handbook is for use by personnel of University of Iowa Health Care. No other use is implied or intended.