The University of Iowa (UIHC)
Department of Pathology
LABORATORY SERVICES HANDBOOK


Phosphatase, Alkaline
Order Code: ALP
Order Form: A-1a General Lab or IPR Req
  Chemistry
6240 RCP
356-3527
Specimen
Plasma
Collection Medium:
Plasma separator tube
Alternate
Collection Media:
Call laboratory for additional acceptable specimen collection 
containers.
Minimum:
2 ml whole blood in light green top tube or 1 microtube for pediatric 
patients
Testing Schedule:
24 hrs/day, 7 days a week, including holidays.
Analytic Time:
2 hours (upon receipt in laboratory)
Reference Range:
Males: 40-129 u/l; females: 35-104 u/l
(higher in children and adolescents)
Comments:
New assay as of (9/5/02).  See reference range change.
Test
Limitations:
Criterion: Recovery within plus or minus 10% of initial values.

Icterus: No significant interference up to an I Index of 70 (conjugated 
bilirubin) and an I Index of 70 (unconjugated bilirubin) corresponding 
to approximately 70 mg/dl conjugated and 70 mg/dl unconjugated 
bilirubin.

Hemolysis: No significant interference up to an H index of 500 
(approximate hemoglobin concentration: 500 mg/dl).

Lipemia (Intralipid): No significant interference up to an L index of 
2000. There is poor correlation between turbidity and triglycerides 
concentration.

Reference
Glick MR, Ryder KW, Jackson SA. Graphical Comparisons of Interferences 
in Clinical Chemistry Instrumentation. Clin Chem 1986;32:470-474.
Methodology:
Spectrophotometric
CPT Code:
84075

Alphabetic main page

Updated: 06/30/2004

Note: The information contained in this handbook is for use by personnel of University of Iowa Health Care. No other use is implied or intended.