The University of Iowa (UIHC)
Department of Pathology
LABORATORY SERVICES HANDBOOK


Lipase
Order Code: LPSE
Order Form: A-1a General Lab or IPR Req
  Chemistry
6240 RCP
356-3527
Specimen
Plasma
Collection Medium:
Plasma separator tube
Alternate
Collection Media:
Call laboratory for additional acceptable specimen collection 
containers.
Minimum:
2 ml whole blood from light green top tube or two microtubes for 
pediatric patients.
Testing Schedule:
24 hrs/day, 7 days a week, including holidays.
Analytic Time:
1 hour (upon receipt in laboratory)
Reference Range:
13-60 U/L
Comments:
Effective 8/23/06, reference range changed to 13-60 U/L.
From 9/9/99 to 8/22/06, reference range was 16-63 U/L.
Prior to 9/9/99, reference range was up to 190 U/L.
Contact the Clinical Chemistry Laboratory at 356-3527 with questions.
Test
Limitations:
Criterion: Recovery within plus or minus 10% of initial values at a 
lipase activity of 50 U/l.

Icterus: No significant interference up to an I index of 60 
(approximate conjugated and unconjugated bilirubin concentration: 60 
mg/dl).

Hemolysis: No significant interference up to an H index of 500 
(approximate hemoglobin concentration: 500 mg/dl)

Lipemia (Intralipid): No significant interference up to an L index of 
1000 (approximate triglycerides concentration: 2000 mg/dl). There is 
poor correlation between turbidity and triglycerides concentration.

References:
Glick MR, Ryder KW, Jackson SA. Graphical Comparisons of Interferences 
in Clinical Chemistry Instrumentation. Clin Chem 1986;32:470-474.

Kaiser H. Fresenius Zeitschr Anal Chem 1965;209: 1-18.
Methodology:
Spectrophotometric
CPT Code:
83690

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Updated: 08/25/2006

Note: The information contained in this handbook is for use by personnel of University of Iowa Health Care. No other use is implied or intended.