The University of Iowa (UIHC)
Department of Pathology
LABORATORY SERVICES HANDBOOK


Lactic Dehydrogenase (LD)
Order Code: LD
Order Form: A-1a General Lab or IPR Req
  Chemistry
6240 RCP
356-3527
Specimen
Plasma
Collection Medium:
Plasma separator tube
Alternate
Collection Media:
Call laboratory for additional acceptable specimen collection 
containers.
Minimum:
2 mls whole blood in light green top tube or 2 microtubes for pediatric 
patients
Testing Schedule:
24 hrs/day, 7 days a week, including holidays.
Analytic Time:
2 hours (upon receipt in laboratory)
Reference Range:
Adult Males   135-225 u/l
Adult Females 135-214 u/l

Pediatric Reference Ranges
                   Male          Female
0-31 days          125-735 u/l   145-765 u/l
31 days-1 year     170-450       190-420
1 year-4 years     155-345       165-395
4 years-7 years    155-345       135-345
7 years-10 years   145-300       140-280
10 years-13 years  120-290       120-260
13 years-16 years  120-290       100-275
16 years-19 years  105-235       105-230
Comments:
Avoid hemolysis.  False elevations may occur in specimens which are not 
processed promptly to separate serum from RBC's.
Test
Limitations:
Criterion: recovery plus or minus 10% of initial values

Plasma
Icterus: No significant interference up to an I index of 60 
(approximate conjugated and unconjugated bilirubin concentration: 60 
mg/dl).

Hemolysis: RBC contamination elevates results; interference if H index 
is greater than or equal to 20.

Lipemia (Intralipid):  No significant interference up to an L index of 
1000.

There is poor correlation between turbidity and triglycerides 
concentration. Highly lipemic samples may cause ABS! flags.
Methodology:
Enzymatic
CPT Code:
83615

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Updated: 09/14/2006

Note: The information contained in this handbook is for use by personnel of University of Iowa Health Care. No other use is implied or intended.