MADS+: discovery of differential splicing events from Affymetrix exon junction array data

Mutually exclusive exons have one of two exons retained in the isoforms, but not both.

HJAY_Plot_mx.pdf contains the graphs of alternative 3'/5' splice site exons. The red lines in the figure represent the gene expression indexes. The blue lines in the figure represent the probe intensities.

 

For mutually exclusive exons, up_juc, down_juc and exon_1 in the first row are upstream exon-exon junction probeset, downstream exon-exon junction probeset and exon probeset for the first exon. The up_juc, down_juc and exon_2 in the second row are for the second exon.

 

The numbers below up_juc, down_juc, exon_1 and exon_2 are the p values for each probeset with directions. Positive p values indicate more inclusion levels in second group of samples. Negative p values indicate less inclusion levels in second group of samples.

 

HJAY_excel_mx.txt contains the gene names, coordinates and p values.

HJAY_gene_ID HJAY_cluster_ID Gene_symbol Exon1_upstream_junction_ ID [p value, direction] Exon1_downstream junction_ID [p value, direction] Exon1_ID [p value, direction] Exon2_upstream_junction_ID [p value, direction] Exon2_downstream_junction_ID [p value, direction] Exon2_ID [p value, direction] Gene_symbol Exon1_ID Exon1_chromosome Exon1_strand Exon1_start Exon1_stop Gene_symbol Exon2_ID Exon2_chromosome Exon2_strand Exon2_start Exon2_stop
812601 TC0700223 AKGDH,E1k,OGDC,OGDH JUC0700015860 [6.2381182782900002e-27, '+'] JUC0700016252 [9.8762033121000007e-31, '+'] PSR070001884 [1.2063670056700001e-14, '+'] JUC0700016115 [9.1246318895100001e-11, '-'] JUC0700016081 [2.9825678989699999e-05, '-'] PSR070001885 [3.0346100124800003e-10, '-'] AKGDH 283254 chr7 + 44653568 44653658 AKGDH 685841 chr7 + 44653781 44653883