MADS+: discovery of differential splicing events from Affymetrix exon junction array data

Alternative 3'/5' splice site is the inclusion or skipping of the extended part of the exon.

HJAY_Plot_alt35.pdf contains the graphs of alternative 3'/5' splice site exons. The red lines in the figure represent the gene expression indexes. The blue lines in the figure represent the probe intensities.

 

For alternative 3'/5' splice sites, shorter_juc=exon-exon junction probeset of shorter isoform, longer_juc=exon-exon junction probeset of longer isoform, shorter_ex=exon probeset of shorter isoform and longer_ex=exon probeset of longer isoform. In which, shorter exon-exon junction probeset and shorter exon probeset are probesets for the shorter isoform of alternative 3'/5' splice sites. Longer exon-exon junction probeset and longer exon probeset are the probesets for the extended isoform of alternative 3'/5' splice sites.

 

The numbers below shorter_juc, longer_juc, shorter_ex and longer_ex are the p values for each probeset with directions. Positive p values indicate more inclusion levels in second group of samples. Negative p values indicate less inclusion levels in second group of samples. 

 

HJAY_excel_alt35.txt contains the gene names, coordinates and p values in spreadsheet.

HJAY_gene_ID HJAY_cluster_ID Gene_symbol Shorter_junction_ID [p value, direction] Longer_junction_ID [p value, direction] Shorter_exon_ID [p value, direction] Longer_part_ID [p value, direction] Gene_symbol HJAY_Exon_ID Exon_chromosome Exon_strand Exon_start Exon_stop
804129 TC0200787 FHOD2,FLJ37546,FMNL2 JUC0200050597 [2.4799470123000001e-11, '+'] JUC0200050957 [6.5501114893099999e-20, '-'] PSR020006434 [0.43354032024599998, '-'] PSR020006435 [9.3082466055799998e-17, '-'] FHOD2 159252 chr2 + 153210240 153210287