Lynda Ostedgaard, PhD Research Scientist University of Iowa Roy J and Lucille A Carver College of Medicine Department of Internal Medicine 500 EMRB Iowa City IA 52242 PHONE: 319 335 6540 FAX: 319 335 7623 lynda-ostedgaard@uiowa.edu

Publications:

Spaziani, E., L.S. Ostedgaard, W.H., Vensel, and J.P. Hegmann, 1981. The molt cycle of the crab, Cancer antennarius.   Computer aided staging. J. Exp. Zool. 218:195-202.

Spaziani, E., L.S. Ostedgaard, W.H. Vensel, and J.P. Hegmann, 1982. Effect of eyestalk   removal in crabs, Cancer antennarius.   Relation to normal premolt. J.Exp. Zool. 221:323-328.

Vensel, W.H., E. Spaziani, and L.S. Ostedgaard, 1984.   Cholesterol turnover and   ecdysone content in tissues of normal and de-eyestalked crabs, Cancer antennarius.   J. Exp. Zool 229:383-392.

Stay, B., L.S. Ostedgaard, S.S. Tobe, A. Strambi, and E. Spaziani, 1984.   Ovarian and hemolymph titers of ecdysteroid during the gonadotropic cycle in Diploptera punctata.   J. Insect Physiol 30:643-652.

Fomon, S.J., M. Janghorbandi, B.T.G. Ting, E.E. Ziegler, R.R. Rogers, S.E., Nelson, L.S.Ostedgaard, and B.B. Edwards, 1988.   Erythrocyte incorporation of ingested 58-iron by infants.   Pediatric Research 24:20-24.

Gregory, R.J., S.H. Cheng, D.P. Rich, J. Marshall, S.Paul, K. Hehir, L. Ostedgaard, K.W. Klinger, M.J. Welsh, and A.E. Smith, 1990.   Expression and characterization of the cystic fibrosis transmembrane conductance regulator. Nature 347:382-386.

Ostedgaard, L.S., M.L. Jennings, L.P. Karniski, and V.L. Schuster, 1991.   A 45 kDa protein antigenically related to Band 3 is selectively expressed in kidney mitochondria. PNAS 88:981-985.

Welsh, M.J., M.P. Anderson, D.P. Rich, L.S. Ostedgaard, R.J. Gregory, S. Cheng, and A.E. Smith.   Cystic Fibrosis, CFTR, and Abnormal Electrolyte Transport.   In: Cystic Fibrosis (P.B. Davis, ed.).   Lung Biology   in Health and Disease Series (D. L'enfant, ed.)   Marcel Dekker, Inc. 1991.

Denning, G.M., L.S. Ostedgaard, S.H. Cheng, A.E. Smith, and M.J. Welsh, 1992.   Localization of cystic fibrosis transmembrane conductance regulator in chloride secretory epithelia.   J Clin Invest 89:339-349.

Tilly, B.C., M. Winter, L.S. Ostedgaard, C. O'Riordan, A.E. Smith, and M.J. Welsh, 1992. cAMP-dependent protein kinase activation of CFTR chloride channels in planar lipid bilayers.   J Biol Chem 267 (14): 9470-9473.

Welsh, M.J., M.P. Anderson, D.P. Rich, H.A. Berger, G.M. Denning, L.S. Ostedgaard, D.N. Sheppard, S. Cheng, R.J. Gregory, and   A.E. Smith, 1992.   Cystic Fibrosis Transmembrane Conductance Regulator: A Chloride Channel with Novel Regulation. Neuron 8:821-829.

Welsh, M., M.P. Anderson, D.P. Rich, H.A. Berger, G.M. Denning, L.S. Ostedgaard, and D.N. Sheppard.   Abnormalities of Airway Epithelial Chloride Transport in Cystic Fibrosis.   In: Airway Secretion: Physiological Bases for the Control of Mucus Hypersecretion. (T.Takishima, Ed.), Marcel Dekker, Inc., N.Y., 1994, pp. 513-526.

Ostedgaard, L.S., D.M. Shasby, and M.J. Welsh, 1992.   Staphylococcus   aureus   alpha toxin permeabilizes the basolateral membrane of a Cl- secreting epithelium.   Amer J Physiol 7 (1):L104-112.

Denning, G.M., L.S. Ostedgaard, and M.J. Welsh, 1992.   Abnormal localization of cystic fibrosis transmembrane conductance regulator in primary cultures of CF airway epithelia. J Cell Biol 118:551-559.

Ostedgaard, L.S. and M.J. Welsh, 1992. Partial purification of the cystic fibrosis transmembrane conductance regulator.   Jour Biol Chem 267 (36):26142-26149.

Sheppard, D.N., D.P. Rich, L.S. Ostedgaard, R.J. Gregory, A.E. Smith, and M.J. Welsh, 1993. Mutations in CFTR associated with mild disease form Cl - channels with altered pore properties.   Nature 362:160-164.

Teem, J.L., H.A. Berger, L.S. Ostedgaard, D.P. Rich, L.-C. Tsui, and M.J. Welsh, 1993. Identification of revertants for the cystic fibrosis ?F508 mutation using STE6/CFTR chimeras in yeast.   Cell 73:335-346.

Welsh, M.J., G.M. Denning, L.S. Ostedgaard, and M.P. Anderson, 1993.   Dysfunction of CFTR bearing the ?F508 mutation. J. Cell Sci, Suppl 17:235-239.

Marshall, J., S. Fang, L.S. Ostedgaard, C.R. O'Riordan, D. Ferrara, J.F. Amara, H. Hoppe IV, R. K. Scheule, M.J. Welsh, A.E. Smith, and S. H. Cheng, 1994. Stoichiometry of recombinant cystic fibrosis transmembrane conductance regulator in epithelial cells and its functional reconstitution into cells in vitro.   Jour Biol Chem 269:2987-2995.

Sheppard, D.N., M.R. Carson, L.S. Ostedgaard, G.M. Denning, and M.J. Welsh, 1994.   Expression of CFTR in a model epithelium.   Amer J Physiol 266:L405-L413.

Sheppard, D.N., L.S. Ostedgaard, D.P. Rich, and M.J. Welsh, 1994.   The amino-terminal portion of CFTR forms a regulated Cl - channel. Cell 76:1091-1098.

Sheppard DN, Ostedgaard LS, Winter MC, and Welsh MJ., 1995. Mechanism of Dysfunction of Two Nucleotide-Binding Domain Mutations in CFTR That are Associated with Pancreatic Sufficiency. EMBO J, 14:876-883.

Cotten JF, Ostedgaard LS, Carson MR, and Welsh MJ., 1996.   Effect of Cystic Fibrosis-associated Mutations in the Fourth Intracellular Loop of Cystic Fibrosis Transmembrane Conductance Regulator. J. Biol. Chem., 271:21279-21284.

Ostedgaard LS, Rich DP, DeBerg LG, and Welsh MJ.   1997.   Association of Domains within the Cystic Fibrosis Transmembrane Conductance Regulator. Biochemistry, 36:1287-1294.

Sheppard, D.N. and Ostedgaard, L.S., 1996. Understanding how cystic fibrosis mutations cause a loss of Cl - channel function.   Mol. Med. Today July 1996: 290.

Hall, R.A., L. S. Ostedgaard, R. T. Premont, JT. Blitzer, N. Rahman, M.J. Welsh, and R. J. Lefkowitz, 1998.   A C-terminal motif found in the b 2-adrenergic receptor, P2Y1 receptor and cystic fibrosis transmembrane conductance regulator determines binding to the Na + /H + exchanger regulatory factor family of PDZ proteins.   PNAS 95:8496-8501.

Ostedgaard, L.S., B. Zeiher, and M.J. Welsh, 1999.   Processing of CFTR bearing the P574H mutation differs from wild-type and D F508-CFTR.   J Cell Sci 112:2091-2098.

Ostedgaard, L.S., O. Baldursson, D.W. Vermeer, M.J. Welsh, and A.D. Robertson, 2000. A Functional R Domain from CFTR is Predominantly Unstructured in Solution.   PNAS 97 (10):5657-5662.

Baldursson O, Ostedgaard LS, Rokhlina T, Cotten JF, and Welsh MJ.   CFTR Cl - Channels with R Domain Deletions and Translocations Show Phosphorylation-Dependent and Independent Activity.   J. Biol. Chem. 276 :1904-1910, 2001.

Ostedgaard LS, Baldursson O, and Welsh MJ. Regulation of the Cystic Fibrosis Transmembrane Conductance Regulator Cl - Channel by its R Domain. J. Biol.Chem. 276 :7689-7692, 2001.

Ostedgaard LS, Zabner J, Vermeer DW, Tokhlina T, Karp PH, Stecenko AA, Randak C, and Welsh MJ. CFTR With a Partially Deleted R Domain Corrects the Cystic Fibrosis Chloride Transport Defect in Human Airway Epithelia In Vitro and in Mouse Nasal Mucosa In Vivo . Proc. Natl. Acad. Sci. USA, 99: 3093-3098, 2002.

Ostedgaard LS, Randak C, Rokhlina T, Karp P, Vermeer D, Ashbourne Excoffon KJ, and Welsh MJ. Effects of C-Terminal Deletions on CFTR Function in Cystic Fibrosis Airway Epithelia. Proc. Natl. Acad. Sci. USA, 100 :1937-1942, 2003 .