The University of Iowa Carver College of Medicine

Proteomics Facility

Proteomics Facility

Work in the Proteomics Facility

The Carver College of Medicine Proteomics Facility will be opening in 2007 and is to be located on the third floor of Eckstein Medical Research Building. The Facility has had two major equipment upgrades with the acquisition of a Thermo LTQ XL linear ion trap mass spectrometer with ETD capabilities. In addition, an Eksigent nanoHPLC has been incorporated into this instrument.

The College anticipates both "discovery" proteomics and in-depth analysis of peptides for post-translational modifications can be performed. An excellent review of methods for phosphopeptide analysis can be found through Thermo. A full proteomics software package has been provided with this instrumentation. The Facility has also acquired a Progest automated tryptic digester from Genomic Solutions. This will allow overnight typsin digestion of up to 90 gel samples.

Following the appointment of a facility director, CCOM anticipates seeking faculty assistance in submitting a large instrumentation grant to the National Center for Research Resources for additional mass spectrometry instrumentation.