The University of Iowa Carver College of Medicine

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Reagents

• Hoechst 33258 (Molecular Probes cat# H3569, 10mg/ml in H₂O
• Deionized H₂O

Stock

• 500µg/ml in DI H₂O (1425µl DI H₂O + 75µl 10mg/ml Hoechst)

Staining

1. Dilute stock 125:1 in cell suspension for final dilution of 4µg/ml (e.g. add 8µl of stock solution to 1ml of cell suspension). Add just before running on flow cytometer.