The University of Iowa Carver College of Medicine

Flow Cytometry Facility

Hoechst for Viability

Reagents

  • Hoechst 33258 (Molecular Probes cat# H3569, 10mg/ml in H2O)
  • Deionized H2O

Stock

  • 250µg/ml in DI H2O (975µl DI H2O + 25µl 10mg/ml Hoechst for 1.0ml of stock)

Staining

Dilute stock 62.5:1 in cell suspension for final dilution of 4µg/ml (e.g. add 16µl of stock solution to 1ml of cell suspension). Add just before running on flow cytometer.