The University of Iowa Carver College of Medicine
Hoechst for Viability
- Hoechst 33258 (Molecular Probes cat# H3569, 10mg/ml in H2O)
- Deionized H2O
- 250µg/ml in DI H2O (975µl DI H2O + 25µl 10mg/ml Hoechst for 1.0ml of stock)
Dilute stock 62.5:1 in cell suspension for final dilution of 4µg/ml (e.g. add 16µl of stock solution to 1ml of cell suspension). Add just before running on flow cytometer.