The University of Iowa Carver College of Medicine

Flow Cytometry Facility

Propdium Iodide for Viability

Reagents

  • Propidium Iodide (Invitrogen cat# P1304MP 100mg powder)
  • 1X PBS

Stock

  • 50µg/ml in 1X PBS

Staining

Dilute stock 50:1 in cell suspension for final dilution of 1µg/ml (e.g. add 20µl of stock solution to 1ml of cell suspension). Add just before running on flow cytometer.