The University of Iowa Carver College of Medicine

Flow Cytometry Facility

Flow Cytometry Protocols

Preparing Cell Samples for Flow Cytometry

Detaching Adherent Cells from Tissue Plates without Trypsin

Staining for Viability

Cell Fixation

Disaggregation of Clumped Cells

Immunofluorescence Staining

Calcium Flux

Apoptosis

DNA Content and Cell Cycle

Intracellular Staining

Links to Other Flow Cytometry Facility Protocols