The University of Iowa Carver College of Medicine
DAPI Staining for DNA Content
- 10µg/ml BAPI (4'-6-diamidino-2-phenylindole dihydrocholride) in PBS with 0.6% NP40 (Nonidet P40)
- Centrifuge 1 x 106 cells to a pellet and carefully remove supernatant.
- Loosen pellet.
- Resuspend pellet in 1ml of DAPI staining solution.
- Keep on ice until run on flow cytometer.