The University of Iowa Carver College of Medicine

Flow Cytometry Facility

DAPI Staining for DNA Content

Reagents

  • 10µg/ml BAPI (4'-6-diamidino-2-phenylindole dihydrocholride) in PBS with 0.6% NP40 (Nonidet P40)

Staining

  1. Centrifuge 1 x 106 cells to a pellet and carefully remove supernatant.
  2. Loosen pellet.
  3. Resuspend pellet in 1ml of DAPI staining solution.
  4. Keep on ice until run on flow cytometer.