The University of Iowa Carver College of Medicine

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Reagents

• 10µg/ml BAPI (4'-6-diamidino-2-phenylindole dihydrocholride) in PBS with 0.6% NP40 (Nonidet P40)

Staining

1. Centrifuge 1 x 10⁶ cells to a pellet and carefully remove supernatant.
2. Loosen pellet.
3. Resuspend pellet in 1ml of DAPI staining solution.
4. Keep on ice until run on flow cytometer.