The University of Iowa Carver College of Medicine

Flow Cytometry Facility

LSR II

LSR II
  • Three Excitation Sources
  • Solid state laser at 488 nm
  • HeNe laser at 633 nm
  • Solid state laser at 405 nm (violet)
  • Pulse Processing
  • Pulse height, width, and area available for all channels
  • Fluorochrome emission overlap compensation can be done at any time during or after data acquisition
  • Pulse trigger can be combination of any channels using boolean logic
  • Ratio of any two signals
  • 10 Channels Plus Time
  • 8 fluorescent channels
  • 2 light scatter channels
  • Kinetics