The University of Iowa Carver College of Medicine

Flow Cytometry Facility

BioRad BioPlex

BioRad BioPlex

The BioRad Bio-Plex will significantly reduce time and labor for quantitative bioassays. Among its many applications, the Bio-Rad Bioplex multiplexing system allows investigators to:

  • Perform up to 100 ELISA like assays simultaneously using only 12ul of serum.
  • Analyze 86 samples plus 10 standards and controls in approximately 1 hour.
  • Quantitatively assay cytokines (ELISA), cellular signaling proteins (Western), and gene expression.
  • Useful for investigations that involve the study of protein expression, genomics, or immunodiagnostics.

Example assays with descriptions:

Cytokine assays are multiplexable bead assays that simultaneously quantitate human, mouse, or rat cytokines in diverse matrices, including cell culture supernatants, serum, or plasma. The Bio-Plex allows profiling up to 27 human, 23 mouse, or 9 rat cytokines in a single 12 Ál serum sample.

Phosphoprotein assays are bead-based multiplexable assays that directly detect phosphorylated proteins in lysates derived from cell culture or tissue samples. Using 96-well filter-plate format assays, it is possible to profile the specific phosphorylation state of multiple proteins with as little as 25 Ál of lysate.

A cytokine kit and analyte finder is available at the Luminex website.

A list of all Luminex capable assays and companies producing assay kits is available at the Luminex website.

For more information, please contact the Flow Cytometry Facility or visit the Luminex website at http://www.luminexcorp.com/.