The University of Iowa Carver College of Medicine

BioRad BioPlex

The BioRad Bio-Plex will significantly reduce time and labor for quantitative bioassays. Among its many applications, the Bio-Rad Bioplex multiplexing system allows investigators to:

Perform up to 100 ELISA like assays simultaneously using only 12ul of serum.
Analyze 86 samples plus 10 standards and controls in approximately 1 hour.
Quantitatively assay cytokines (ELISA), cellular signaling proteins (Western), and gene expression.
Investigations that involve the study of protein expression, genomics, or immunodiagnostics, will benefit from the high throughput and ease of use that this instrument offers.

Example assays with descriptions from the manufacturers:

Panomics QuantiGene Plex is a gene expression profiling assay that can multiplex from 3 to 30 targets in a single well. The assay measures gene specific RNA directly from cell lysates and tissue homogenates, so there is no need for RNA purification, cDNA synthesis, or PCR. Since the assay is hybridization based, it is extremely robust and reproducible and works with lysates, homogenates, whole blood, or Total RNA. The workflow is similar to running an ELISA. Currently, there are over 150 different panels customers can access immediately or custom panels are available in 4 weeks.

BioRad Bio-Plex cytokine assays are multiplexable bead assays that simultaneously quantitate human, mouse, or rat cytokines in diverse matrices, including cell culture supernatants, serum, or plasma. Bio-Plex allows you to profile up to 27 human, 23 mouse, or 9 rat cytokines in a single 12 µl serum sample. Cytokine panels are premixed, whether you select preconfigured or custom-mixed (x-Plex) panels containing only the analytes you want.

BioRad Bio-Plex phosphoprotein assays are bead-based multiplexable assays that directly detect phosphorylated proteins in lysates derived from cell culture or tissue samples. Using these 96-well filter-plate format assays, you can profile the specific phosphorylation state of multiple proteins with as little as 25 µl of lysate. Choose from a broad assay selection and use the assays individually to analyze samples for one target at a time, or combine them to create a multiplex assay to test for a specific set of phosphoproteins in a single sample. Custom-premixed multiplex assays are available through Bio-Rad's online x-Plex assay service.

A list of assays and companies producing assay kits is available at the Luminex website.

For more information, please contact the Flow Cytometry Facility or visit the Luminex website at http://www.luminexcorp.com/.