The University of Iowa Carver College of Medicine

Flow Cytometry Facility

Miltenyi autoMACS

Miltenyi autoMACS
  • Magnetic based cell separator
  • Useful for pre-enrichment before fluorescence-based cell sorting
  • Capacity
  • 4 x 109 total cells
  • 1 x 107 per 100 µl cell concentration
  • 2 x 108 total labeled cells
  • 1 x 105 minimum number of cells
  • 500 µl minimum sample volume
  • For positive selection, instrument puts separated cells in 2 ml
  • For negative selection, instrument puts separated cells in 2 ml plus the original volume
  • Sort Programs
  • Positive or negative selection for all combinations of high and low antigen expression for recovery or purity