The University of Iowa Carver College of Medicine
Flow Cytometry Facility
Miltenyi autoMACS
- Capacity
- 4 x 109 total cells
- 1 x 107 per 100 µl cell density
- 2 x 108 total labeled cells
- 1 x 105 minimum number of cells
- 500 µl minimum sample volume
- For positive selection, instrument puts separated cells in 2 ml
- For negative selection, instrument puts separated cells in 2 ml plus the original volume
- Sort Programs
- Positive or negative selection for all combinations of high and low antigen expression for recovery or purity

