Borrelia burgdorferi Antibodies, IgG & IgM by Western Blot, Serum

Borrelia burgdorferi Antibodies, IgG & IgM by Western Blot

Order Code:

LYMEWB

Specimen:

Serum

Collection Medium:

Red top tube

Minimum:

Preferred Minimum: 1 mL serum in a red top tube.
Absolute Minimum: 0.1 mL serum in a red top tube.

Rejection Criteria:

CSF or plasma. Severely lipemic, heat-inactivated, contaminated, or hemolyzed specimens.

Turn Around Time:

2 days upon receipt at reference laboratory

Reference Range:

Negative

Interpretive Data:

IgG: For this assay, a positive result is reported when any 5 or more of the following 10 bands are present: 18, 23, 28, 30, 39, 41, 45, 58, 66, or 93 kDa. All other banding patterns are reported as negative.

IgM: For this assay, a positive result is reported when any 2 or more of the following bands are present: 23, 39, or 41 kDa. All other banding patterns are reported as negative.

Test Limitations:

Per CDC guidelines, if ELISA test result is NEGATIVE, Western blot should not be performed.

This test should be used for confirmation of an equivocal or positive Borrelia burgdorferi total antibodies, IgG and/or IgM test performed on patients less than four weeks after appearance of erythema migrans.

Sample Processing:

Label transport tube with two patient identifiers, date and time of collection.
Aliquot serum into labeled container and cap.
Centrifuge at 3000 RPM for 10 minutes.
Centrifuge within one hour of draw time.

Sample Storage:

Refrigerate serum 2-8°C.

Transport Instructions:

Place requisition into outside pocket of bag.
Place specimen into zip-lock type bag, seal bag.
Use refrigerated coolant packs.

CPT Code:

86617 Lyme IgG; 86617 Lyme IgM