Epidermal Growth Factor Receptor (EGFR) Sequencing
| Order Code: | EGFR |
| Order Form: | Molecular Oncology Requisition |
Specimen:
Formalin fixed, paraffin embedded tissue block and 10 unstained 5-10 μM slides at 20-25°C and an H&E slide of the tissue
Minimum:
Tumor cells more than 50% of the total tissue and greater than
10mm2 in surface area on the block
Rejection Criteria:
Specimens fixed in B5 fixative or that have been decalcified will not
be accepted. Tumor specimens containing less than 50% tumor cells or
are less than 10mm2 in area may be unacceptable.
Testing
Schedule:
Weekly
Turn Around
Time:
7-10 working days
Reference Range:
Negative
Methodology:
PCR followed by Fluorescence Capillary Electrophoresis
Instructions:
Submit formalin fixed, paraffin embedded tissue block or 10
unstained
5-10 μM slides and an H&E slide along with the requisition. Please include surgical pathology report and relevant clinical information.
5-10 μM slides and an H&E slide along with the requisition. Please include surgical pathology report and relevant clinical information.
Sample
Processing:
Relevant clinical information must be submitted with specimen in order to provide correct interpretation of test results.
Formalin-fixed, paraffin embedded tissue should be submitted.
Formalin-fixed, paraffin embedded tissue should be submitted.
Sample
Storage:
Tissue Specimen - Freeze immediately and store frozen or send Formalin-fixed paraffin embedded blocks.
Transport
Instructions:
Frozen Tissue - Ship frozen on dry ice. Ship express mail with overnight delivery. Formalin-fixed paraffin embedded blocks ship at ambient temperature.
Place requisition into outside pocket of bag.
Protect block or slides from excessive heat and ship in cooled container during summer months.
Place requisition into outside pocket of bag.
Protect block or slides from excessive heat and ship in cooled container during summer months.
CPT Code:
83890, 83898(x4), 83904(x4), 83907, 83912