bcl-2 PCR Assay for Lymphoma
| Order Code: | BCL2PCR |
| Order Form: | Molecular Oncology Requisition |
Collection Medium:
 | or |  |
| Pink top tube | Yellow top tube (ACD solution A) |
Minimum:
5 mL blood in pink or yellow top, 1 mL bone marrow aspirate in
pink or yellow top, or 3 cu mm tissue. Tissue may be fresh frozen
or paraffin embedded formalin fixed. Body fluids in pink or yellow
top tubes, Lymph node aspirates in RPMI.
Testing
Schedule:
Weekly
Turn Around
Time:
7 working days
Interpretive Data:
Used as an adjunct in initial diagnosis or as a monitor of minimal
residual disease. When used as a monitor of minimal residual disease,
current samples must be analyzed in parallel with a previous diagnostic
sample.
Comments:
Transport fresh tissue on dry ice. Blood, bone marrow and lymph
aspirates should be transported on ice.
Test
Limitations:
Specimens fixed in BS fixative are unsuitable for testing.
Methodology:
Multiplex PCR followed by Fluorescence Capillary Electrophoresis
Sample
Processing:
Label transport tube with two patient identifiers, date and time of collection.
Relevant clinical information must be submitted with specimen in order to provide correct interpretation of test results.
Specimen source is required on requisition for processing.
Tissue specimen should be frozen or processed as a formalin fixed paraffin embedded tissue.
Lavender tube is sent as whole blood, no processing necessary.
Relevant clinical information must be submitted with specimen in order to provide correct interpretation of test results.
Specimen source is required on requisition for processing.
Tissue specimen should be frozen or processed as a formalin fixed paraffin embedded tissue.
Lavender tube is sent as whole blood, no processing necessary.
Sample
Storage:
Blood/Bone Marrow - Room temperature or ambient storage.
Tissue Specimen - Freeze immediately and store frozen or send Formalin-fixed paraffin embedded blocks.
Tissue Specimen - Freeze immediately and store frozen or send Formalin-fixed paraffin embedded blocks.
Transport
Instructions:
Blood - Do not freeze, ship at ambient temperature. Ship express mail with overnight delivery.
Frozen Tissue - Ship frozen on dry ice. Ship express mail with overnight delivery. Formalin-fixed paraffin embedded blocks ship at ambient temperature.
Frozen Tissue - Ship frozen on dry ice. Ship express mail with overnight delivery. Formalin-fixed paraffin embedded blocks ship at ambient temperature.
CPT Code:
83890, 83900, 83901, 83909(x3), 83912; if paraffin block or fresh
tissue is submitted, add 83907.