Specimen:

Urine More information

Collection Medium:

Minimum:

24 hour collection to yield 5 mL aliquot from well mixed 24 hr sample.
Pediatric minimum is 1 mL aliquot from well mixed 24 hr sample.

Delivery Instructions:

Submit specimen to laboratory as soon as possible after
collection period is completed.

Specimen
Instructions:

Record start and end of 24 hour collection on requisition. Urine must 
be refrigerated during collection.

Analytic Time:

2 working days (upon receipt)

Reference Range:

Cortisol (ug/day)
  Female
    3-8 years:  Less than 18 ug/day
    9-12 years: Less than 37 ug/day
   13-17 years: Less than 56 ug/day
   18 years and older: Less than 45 ug/day
  Male
    3-8 years:  Less than 18 ug/day
    9-12 years: Less than 37 ug/day
   13-17 years: Less than 56 ug/day
   18 years and older: Less than 60 ug/day

Cortisol/Cortisone, Ratio*
  Female
    0-11 years: To be determined
    12 years and older: 0.15-0.50
  Male
    0-11 years: To be determined
    12 years and older: 0.15-0.50

Cortisone
  Female
    Value used to calculate Cortisol/Cortisone ratio

Cortisol (ug/g crt)
  Female
    Prepubertal: Less than 25 ug/g crt
    18 years and older: Less than 45 ug/g crt
    Pregnancy: Less than 59 ug/g crt
  Male
    Prepubertal: Less than 25 ug/g crt
    18 years and older: Less than 32 ug/g crt

The urine cortisol-to-creatinine ratio will be reported when the urine 
collection is random, less than 20 hours, or the urine volume is less 
than 400 mL/24 hours.

Comments:

*The ratio of the concentrations of cortisol to cortisone should not
 be evaluated if the cortisol concentration is < 5 ug/L.

Methodology:

Tandem Mass Spectrometry (LC-MS/MS)

CPT Code:

Cortisol 82530
LC-MS/MS 83789

See Additional Information:
Collection and Preservation of 24-Hour Urine Specimens
Urine Tests Requiring Preservatives, Refrigeration or Special Containers