Chronic Lymphocytic Leukemia Flow Cytometry Panel

Epic code: See comments
Order form: Epic Beaker AP Order Label
Flow Cytometry Service
6240 RCP
Flow Cytometry Resident: 467-6008
Flow Cytometry Lab: 467-6009 or 467-6010
Tube Station #260
Specimen(s):
Various
Collection Medium:
Yellow top tube 8.5 mL (ACD solution A)
Alternate Collection Media:
Green top tube 4 mL (Na Heparin) or Lavender top tube 3 mL (EDTA)
Minimum:

Peripheral Blood: 2 mL

 

Bone Marrow: 2–4 mL yellow top tube (ACD solution A)

 

Fluids and Tissue: Dispense sample into vial of RPMI-1640 tissue culture media.

Delivery Instructions:
Keep at room temperature. Do not refrigerate.

Send to tube station #260 or deliver to Specimen Control, 6240 RCP.

Testing Schedule:

Samples will be processed during the hours of 0800–1600 daily. Samples that arrive on weekends or holidays will be triaged and processed during the next business day.

Turn Around Time:
2 days
Reference Range:

The pathologist will provide an interpretative report.

Comments:

LAB8255 for Blood, LAB8284 for Bone Marrow, LAB8254 for Other

Please state the clinical question to be answered in the order. Clinical Pathology resident should be contacted if studies are needed emergently at other times.

 

Antibodies routinely included are: CD2, CD3, CD4, CD5, CD7, CD8, CD10, CD14, CD19, CD20, CD23, CD25, CD38, CD45, CD56, CD103, CD138, CD200, FMC7, Kappa and Lambda. Antibodies will also identify cases of prolymphocytic and Hairy Cell Leukemia.

If clinical or morphologic features suggest a "T" or "NK" lymphocyte disorder, then additional antibody combinations may be performed.

Methodology:

Flow Cytometry-Lysed Whole

CPT Code:
88184 (first antibody), 88185(for each additional antibody) technical; 88189 professional; T cell or NK cell disorder: 88184, 88185(x15) technical; 88188 professional
 
See Additional Information:
Specimens Requiring Immediate Delivery