Specimen:

Whole Blood

Collection Medium:

Alternate
Collection Media:

Light Green top tube (Lithium Heparin)

Minimum:

Adult Preferred Minimum:  Two 4 mL green (sodium or lithium heparin) 
from patient AND two 4 mL green (sodium heparin or lithium heparin) 
from normal control subject drawn at the same time.  Client must supply 
the normal control.

Adult Absolute Minimum:  5 mL whole blood from patient AND 5 mL whole 
blood from control subject drawn at the same time.

Pediatric Minimum:  1 mL patient whole blood and 1 mL control whole 
blood.

Rejection Criteria:

Clotted, ambient, or frozen samples. Samples submitted without a normal 
control drawn at the same time. Samples greater than 24 hours.

Delivery Instructions:

Deliver to laboratory immediately after collection.
CRITICAL REFRIGERATED. The sample must be received within 24 hours of 
collection.  Sample viability is 24 hours only, control and patient 
sample must reach reference laboratory within the 24 hour time-frame.

Suggested time for collection:  Noon-3:00 p.m.  (Bring specimen to 
Specimen Control immediately)

Antigen densities cannot be reported unless a normal control sample is 
also supplied.

Analytic Time:

4 working days

Reference Range:

Component      Reference Interval
% CD11b              By report
CD11b Density        By report
% CD15               By report
CD15 Density         By report
% CD16               By report
CD16 Density         By report
% CD18               By report
CD18 Density         By report

Interpretive Data:

The Neutrophil Receptor Panel measures CD11b, CD15, CD16, and CD18 on 
neutrophils. CD11b is the receptor for the C3bi fragment of complement. 
CD15 is a ligand for E and P selectins.  CD16 is the IgG Fc receptor. 
CD11b is an alpha subunit, and CD18 is the beta subunit of Mac-1, also 
known as CR3. All of these receptors are normally found on neutrophils. 
The percentage of patient neutrophils bearing these receptors is 
reported. In addition, the receptor surface density of each antigen is 
compared to normal neutrophils and expressed as a percent. Values 
outside of the reference interval may correlate with abnormal 
neutrophil function. For example, CD18 is decreased or absent in 
leukocyte adhesion deficiency (LAD) type I and CD15 is decreased or 
absent in LAD type II.

Methodology:

Flow Cytometry

CPT Code:

88184 First marker; 88185 x3 Each additional marker

See Additional Information:
Specimens Requiring Immediate Delivery