The University of Iowa Carver College of Medicine
Core Research Facilities
Gene Targeting Facility
The Gene Targeting Core Facility (GTCF) at the University of Iowa was established with generous support from the Bioscience Initiative and Carver College of Medicine on July 1st, 1998. It is one of the several core facilities operated and supported by the Roy J. and Lucille A. Carver College of Medicine.
The Gene Targeting Core Facility provides technical and research services to investigators on the University of Iowa campus and elsewhere for the generation of gene knockout mouse models. These services include:
- consultations on developing strategies for gene targeting and design of targeting construct,
- generation of targeted ES cells,
- microinjections of targeted ES cells, embryo transfer and generation of chimeras, and
- breeding of chimeras for germline transmission to pass on the designed genomic changes to the next generation.
Over the past nine years, the Gene Transfer Core Facility has worked with more than 40 investigators on this campus on over 180 different research projects and generated more than 50 lines of mutant mice through gene targeting via DNA homologous recombination.